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e selectin cd62e alexa fluor 488  (Novus Biologicals)


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    Novus Biologicals e selectin cd62e alexa fluor 488
    E Selectin Cd62e Alexa Fluor 488, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nb110+85473af488/E-Selectin%2FCD62E+Antibody+(RM0034-9J12)+%5BAlexa+Fluor%C2%AE+488%5D/pmc09243393-106-42-49
    Average 90 stars, based on 1 article reviews
    e selectin cd62e alexa fluor 488 - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: E-Selectin/AAV2/2 Gene Therapy Alters Angiogenesis and Inflammatory Gene Profiles in Mouse Gangrene Model
    Article Snippet: .. Slides were then washed in distilled water and permeabilized with 0.25% Triton-X100 TBS for 15 min. Tissue was incubated with Protein Block (ab64226, Abcam, Cambridge, United Kingdom) for 1 h. Slides were then incubated overnight at 4°C with conjugated primary antibodies, including E-selectin/CD62E Alexa Fluor 488 (5 μg/mL) (NB110-85473AF488, Novus Biologicals, Littleton, CO), CD31/PECAM-1 Alexa Fluor 647 (5 μg/mL) (NB600-1475AF647, Novus Biologicals), KDR/VEGFR-2 Alexa Fluor 647 (5 μg/mL) (NBP1-43300AF647, Novus Biologicals), CD34 AF488 (5 μg/mL) (SC-18917AF488, Santa Cruz Biotechnology, Dallas, TX) CD3 FITC (5 μg/mL) (11-0032-82, eBioscience/Thermo Fisher Scientific), and unconjugated Mac-2/Galectin-3 (5 μg/mL) (125401, BioLegend, San Diego, CA) followed by Alexa Fluor 594 goat anti-rat IgG (2 μg/mL) (A11007, Invitrogen). .. To confirm antibody specificity, isotype-matched negative controls were performed using rat IgG2a, kappa monoclonal antibody (ab18450, Abcam) (5 μg/mL) with Alexa Fluor 594 goat anti-rat IgG (2 μg/mL) as secondary antibody ( ).

    Blocking Assay:

    Article Title: E-Selectin/AAV2/2 Gene Therapy Alters Angiogenesis and Inflammatory Gene Profiles in Mouse Gangrene Model
    Article Snippet: .. Slides were then washed in distilled water and permeabilized with 0.25% Triton-X100 TBS for 15 min. Tissue was incubated with Protein Block (ab64226, Abcam, Cambridge, United Kingdom) for 1 h. Slides were then incubated overnight at 4°C with conjugated primary antibodies, including E-selectin/CD62E Alexa Fluor 488 (5 μg/mL) (NB110-85473AF488, Novus Biologicals, Littleton, CO), CD31/PECAM-1 Alexa Fluor 647 (5 μg/mL) (NB600-1475AF647, Novus Biologicals), KDR/VEGFR-2 Alexa Fluor 647 (5 μg/mL) (NBP1-43300AF647, Novus Biologicals), CD34 AF488 (5 μg/mL) (SC-18917AF488, Santa Cruz Biotechnology, Dallas, TX) CD3 FITC (5 μg/mL) (11-0032-82, eBioscience/Thermo Fisher Scientific), and unconjugated Mac-2/Galectin-3 (5 μg/mL) (125401, BioLegend, San Diego, CA) followed by Alexa Fluor 594 goat anti-rat IgG (2 μg/mL) (A11007, Invitrogen). .. To confirm antibody specificity, isotype-matched negative controls were performed using rat IgG2a, kappa monoclonal antibody (ab18450, Abcam) (5 μg/mL) with Alexa Fluor 594 goat anti-rat IgG (2 μg/mL) as secondary antibody ( ).

    other:

    Article Title: E-Selectin/AAV2/2 Gene Therapy Alters Angiogenesis and Inflammatory Gene Profiles in Mouse Gangrene Model.
    Article Snippet: Tissue was incubated with Protein Block (ab64226, Abcam, Cambridge, United Kingdom) for 1 h. Slides were then incubated overnight at 4◦C with conjugated primary antibodies, including E-selectin/CD62E Alexa Fluor 488 (5μg/mL) (NB110-85473AF488, Novus Biologicals, Littleton, CO), CD31/PECAM-1 Alexa Fluor 647 (5μg/mL) (NB600-1475AF647, Novus Biologicals), KDR/VEGFR-2 Alexa Fluor 647 (5μg/mL) (NBP1-43300AF647, Novus Biologicals), CD34 AF488 (5μg/mL) (SC-18917AF488, Santa Cruz Biotechnology, Dallas, TX) CD3 FITC (5μg/mL) (11-0032-82, eBioscience/Thermo Fisher Scientific), and unconjugated Mac-2/Galectin-3 (5μg/mL) (125401, BioLegend, San Diego, CA) followed by Alexa Fluor 594 goat anti-rat IgG (2μg/mL) (A11007, Invitrogen).



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